Project Information

Reference Number 2020-0324
Project Title Development of Local Eggplant Varieties with Reduced Fruit Browning, and/or Improved Insect Resistance through Editing of Candidate Genes
Project Type Contained Use (Laboratory)
Status Ongoing
Name of Institution International Rice Research Institute
Cooperating Institution None
Supervising IBC International Rice Research Institute (IRRI)
Project Leader(s)

Dr. Desiree M. Hautea

Experimental Facility/Site Institute of Plant Breeding, University of the Philippines, Los Baños
Purpose / Objectives

The objective of this proposal is to perform targeted mutagenesis via CRISPR/Cas9 gene editing of genes related to insect resistance, enzymatic browning, and parthenocarpy in eggplant.

Biosafety measures
  • Restricted access to IPB-BL2 facility. The gate is provided with a padlock and will be locked at all times. All personnel are required to sign in the logbook.
  • All media, regenerants and plantlets will be autclaved and buried in the facility.
  • Bagging of flowers will be done prior to flower opening to prevent unintended transfer of pollen. Any open flowers not bagged will be immediately removed.
  • All plant parts will be disposed in the area. Fruits will be chopped, boiled/autoclaved and buried inside the IPB-BL2 facility. WHole plants will be chopped and buried inside the area.
  • Contingency plans available in case of typhoons, flooding and other possible destructive cases of the IPB-BL2 facility.
Conditions for Approval N/A
Date of Approval (DD-MM-YYYY) 16-05-2020
Date of Completion (DD-MM-YYYY) N/A
Executive Summary

Eggplant (Solanum melongena L.) is one of the most important and widely produced horticultural crops in the Philippines. Eggplant total production estimates surpass that of other vegetable crops, with an average production area of -21,300 hectares valued at Php 2.691B (average from 2009-2018; PSA, 2020). Both pre-harvest and post harvest conditions can adversely influence eggplant production and marketability. Eggplant has high phenolic content (Helmja et al., 2007) and is prone to post-harvest browning, which makes it difficult to preserve and market once it is sliced. Phenolics in eggplant are associated with nutraceutical benefits (Plazas et al., 2013). However, browning hinders the potential for breeding eggplant with increased phenolic content, as an increase in phenolis may also increase browning index (Gramazio et al., 2013; Mishra et al., 2013). In the farm, two of the fruit's principal pests, the eggplant fruit and shoot borer (FSB), Leucinodes orbonalis Guenee, and leafhopper (LH), Amrasca biguttula Ishda, bring about devastation and loss of fruiting plants. Sustainable and effective solutions are critically needed to improve farmer productivity and fruit palatability with minimized environmental risks.