|Project Title||Development of Local Eggplant Varieties with Reduced Fruit Browning, and/or Improved Insect Resistance through Editing of Candidate Genes|
|Project Type||Contained Use (Laboratory)|
|Name of Institution||International Rice Research Institute|
|Supervising IBC||International Rice Research Institute (IRRI)|
Dr. Desiree M. Hautea
|Experimental Facility/Site||Institute of Plant Breeding, University of the Philippines, Los Baños|
|Purpose / Objectives||
The objective of this proposal is to perform targeted mutagenesis via CRISPR/Cas9 gene editing of genes related to insect resistance, enzymatic browning, and parthenocarpy in eggplant.
|Conditions for Approval||N/A|
|Date of Approval (DD-MM-YYYY)||16-05-2020|
|Date of Completion (DD-MM-YYYY)||N/A|
Eggplant (Solanum melongena L.) is one of the most important and widely produced horticultural crops in the Philippines. Eggplant total production estimates surpass that of other vegetable crops, with an average production area of -21,300 hectares valued at Php 2.691B (average from 2009-2018; PSA, 2020). Both pre-harvest and post harvest conditions can adversely influence eggplant production and marketability. Eggplant has high phenolic content (Helmja et al., 2007) and is prone to post-harvest browning, which makes it difficult to preserve and market once it is sliced. Phenolics in eggplant are associated with nutraceutical benefits (Plazas et al., 2013). However, browning hinders the potential for breeding eggplant with increased phenolic content, as an increase in phenolis may also increase browning index (Gramazio et al., 2013; Mishra et al., 2013). In the farm, two of the fruit's principal pests, the eggplant fruit and shoot borer (FSB), Leucinodes orbonalis Guenee, and leafhopper (LH), Amrasca biguttula Ishda, bring about devastation and loss of fruiting plants. Sustainable and effective solutions are critically needed to improve farmer productivity and fruit palatability with minimized environmental risks.