Project Information

Reference Number 2019-0321
Project Title Transformation of Rice with genes for increasing gibberellin production and mesocotyl length
Project Type Confined Test
Status Ongoing
Name of Institution International Rice Research Institute
Cooperating Institution Rothamsted Research
Supervising IBC International Rice Research Institute (IRRI)
Project Leader(s)

Dr. Inez Slamet-Loedin

Experimental Facility/Site Genetic Transformation Laboratory (GTL), Transgenic glasshouse (CL4)
Purpose / Objectives

To transform rice with gene for enhanced production of the plant growth regulator gibberellin in mesocotyl and genes for increased mesocotyl length.

Biosafety measures

Restricted access and movement of transgenic materials.  Storage of transgenic seeds in transgenic seed storage and disposition of samples not needed

Conditions for Approval

a) All activities shall be conducted at the GTL, the Genetics and Genomics Laboratory and the Transgenic Glasshouse at IRRI, Los Baños, Laguna;

b) Measures to limit access to authorized personnel should strictly be observed;

b) The proponent shall submit to the DOST-BC a modified Gantt chart and shall adhere as close as possible to the revised schedule of activities;

c) Any modification in the schedule and additional activities must be communicated to the DOST-BC for information and/or approval;

d) All confinement measures should be in place prior to the commencement of the activities;

e) Signages should be in place indicating that the facility is a restricted/limited access facility and only those personnel approved by the DOST-BC;

f) Any changes in the list of authorized personnel shall be approved by the IRRI-IBC and notification on the same shall be communicated to the DOST-BC for record purposes;

g) Monitoring for biosafety compliance shall supervised by the IRRI-IBC;

h) The IRRI-IBC shall approve request for visits of unauthorized personnel and notification on the same shall be sent to the DOST-BC;

i) The proponent and the IRRI-IBC shall ensure that there are no breach in the biosafety protocol imposed on the project;

j) In case of incident or undue destruction in the facility resulting from unauthorized entry of personnel or breach in containment, the proponent shall implement the biosafety contingency measures to prevent the inadvertent escape of any viable materials to the outside environment;

k) The IRRI-IBC shall submit to the DOST-BC a report on the monitoring activity, within five (5) days after its completion in accordance with the prescribed format; and

l) After completion of the project, all viable materials within the experiment area shall be duly accounted for.

Date of Approval (DD-MM-YYYY) 21-09-2019
Date of Completion (DD-MM-YYYY) N/A
Executive Summary

The most widely used method for cultivating rice involves germinating the seeds in nurseries and transplanting the seedlings to flooded 'paddy' fields. This transplanted rice method accounts for ~55% of global rice cultivation and ~75% of world production. However, in many parts of South and Southeast Asia, farmers are switching to direct seeded rice (DSR) because the availability of labor and water is in decline and their associated costs are rising. A similar transition is also occurring in West Africa. To obtain a good crop using DSR it is imperative that the seeds germinate quickly in the soil and that the seedlings grow vigorously. However, because breeders have focused on developing varieties for transplanting there has been little or no selection for seed vigor. Agronomists have highlighted a need for the development of new rice varieties with improved seed vigor so that they perform better in dry direct seeded conditions.           

The aim of this project is to transform semi-dwarf rice varieties with genes for enhancing the production of the plant growth regulator gibberellin in a specific seedling tissue called the mesocotyl and also for increasing mesocotyl length. The increase in mesocotyl elongation could help seedlings to emerge from the soil when the seed has been planted at a greater depth which is a weakness of current varieties.

Transgenic plants produced will be analyzed for transgenes presence. The DNA or seeds derived from the selected primary transgenic lines will be transported to Rothamsted for further analysis.The site of the contained experiment is the Genetic Transformation Laboratory (GTL) and transgenic glasshouse (CL4) at IRRI, Los Baños, Laguna.   The project is expected to be completed in two years.