Project Information
| Reference Number | 20090237 |
| Project Title | Contained Trial of Selected PRSV-CP Papaya Plants from Candidate Events E-124 and 132 for Further Breeding and Event Selection, Virus Resistance Testing and Molecular Characterization |
| Project Type | Contained Use (Greenhouse) |
| Status | Completed |
| Name of Institution | University of the Philippines - Los Banos |
| Cooperating Institution | N/A |
| Supervising IBC | University of the Philippines-Los Banos (UPLB) |
| Project Leader(s) | Dr. Desiree M. Hautea |
| Experimental Facility/Site | Institute of Plant Breeding, Crop Science Cluster, College of Agriculture, UP Los Baños, College, Laguna |
| Purpose / Objectives | Main Objective Specific Objectives • To conduct virus resistance screening test to assess and confirm the PRSV reaction of selected plants and/or progenies in confined trials 1 and 2 • To generate further molecular data of selected highly resistant PRSV event/line(s) • To establish generational stability of gene insert and PRSV resistance trait of selected events and lines |
| Biosafety measures | N/A |
| Conditions for Approval | • That the approved activities will be conducted at the following facilities of the Institute of Plant Breeding, UPLB: 1) micropropagation of test plants – P2 tissue culture laboratory of the BL2 Headhouse; 2) PRSV resistance screening of seedlings from selected transgenic events – BL2 greenhouse/screenhouse and 3) molecular analysis of leaf samples from selected events and lines – P1 laboratory (IPB Genetics Laboratory) and only authorized personnel are allowed inside the facilities • That only authorized personnel be allowed access to the above-mentioned facilities • That the proponents adhere closely to the schedule of activities • That modifications in the planting schedule and other activities could be made with the concurrence of IBC and DOST-BC • That papaya plants shall be cut down when the plants start to show severe yellowing’ little leaf formation, leaf deformation and reduction in canopy size (50-75%) of the plants to limit the inoculum level • That the gadget used in cutting the infected papaya plants be treated with bleach or heat-treated before using again • That all plant materials that have been cut down be disposed by burying in a pit inside the BL2 greenhouse • That the DOST-BC and the Plant Quarantine Services, Bureau of Plant Industry (PQS-BPI) should be informed in advance of any visitations by unauthorized persons • That all plants and viable materials within the experiment shall be accounted for • That the biosafety contingency plan be submitted before the conduct of the experiment • That a soft copy of the project proposal be submitted for for record keeping • That the additional requirements that the DOST-BC may impose, as necessary, during the experiment period be complied with |
| Date of Approval (DD-MM-YYYY) | 14-03-2009 |
| Date of Completion (DD-MM-YYYY) | 11-2011 |
| Executive Summary | N/A |