Project Information

Reference Number 20090239
Project Title Production and evaluation of transgenic rice using candidate genes for C4 photosynthesis
Project Type Contained Use (Greenhouse)
Status Ongoing
Name of Institution International Rice Research Institute
Cooperating Institution Dr. Zhen Zhu
Supervising IBC International Rice Research Institute (IRRI)
Project Leader(s)

Dr. Inez Slamet-Loedin

Experimental Facility/Site All plant transformation experiments will be conducted in the Rice Biotechnology Laboratory, Plant Breeding, Genetics and Biotechnology (PBGB), IRRI, Los Banos, Laguna • Phenotypic analysis will be performed at IRRI CL4 and transgenic screenhouse
Purpose / Objectives

Production of transgenic rice using candidate genes for C4 photosynthesis.

Evaluation and functional assessment of candidate genes

Biosafety measures

• All vectors will be kept in the plasmid collection of the Rice Biotechnology Laboratory, IRRI and/or Molecular Physiology, Cambridge.

• The tested plants will be kept in isolation in the CL4 transgenic screenhouse to prevent access of unauthorized personnel.

• Plant materials for disposal will be placed in sealed plastic bags.

• Plants grown in CL4 will be destroyed and the pots and soil will be autoclaved at high temperature and high pressure.

• Plant materials from the screenhouses (e.g. CS01 and others) will be disposed through appropriate disposal system in compliance with biosafety and related regulations in the Philippines.

• Access to the screenhouses would be for authorized staff only.

• A barcode entry system is applied in the screenhouses to record the entry of personnel; a logbook is available for visitors not having IRRI access card system.

• Screenhouse facilities are adequate to prevent entry of rats, birds, etc.

• Transgenic seeds harvested from CL4 and the screenhouses will be kept in hermetically-sealed aluminum foil packages and stored in locked refrigerators designated for storage of transgenic seeds in Rice Biotechnology Laboratory, IRRI.

• Movement of all materials will be done in compliance of all relevant biosafety and phytosanitary requirements of the Philippines.

Conditions for Approval N/A
Date of Approval (DD-MM-YYYY) 18-04-2009
Date of Completion (DD-MM-YYYY) N/A
Executive Summary N/A