Dr. Inez Slamet-Loedin

Production of transgenic rice using candidate genes for C4 photosynthesis.

Evaluation and functional assessment of candidate genes

• All vectors will be kept in the plasmid collection of the Rice Biotechnology Laboratory, IRRI and/or Molecular Physiology, Cambridge.

• The tested plants will be kept in isolation in the CL4 transgenic screenhouse to prevent access of unauthorized personnel.

• Plant materials for disposal will be placed in sealed plastic bags.

• Plants grown in CL4 will be destroyed and the pots and soil will be autoclaved at high temperature and high pressure.

• Plant materials from the screenhouses (e.g. CS01 and others) will be disposed through appropriate disposal system in compliance with biosafety and related regulations in the Philippines.

• Access to the screenhouses would be for authorized staff only.

• A barcode entry system is applied in the screenhouses to record the entry of personnel; a logbook is available for visitors not having IRRI access card system.

• Screenhouse facilities are adequate to prevent entry of rats, birds, etc.

• Transgenic seeds harvested from CL4 and the screenhouses will be kept in hermetically-sealed aluminum foil packages and stored in locked refrigerators designated for storage of transgenic seeds in Rice Biotechnology Laboratory, IRRI.

• Movement of all materials will be done in compliance of all relevant biosafety and phytosanitary requirements of the Philippines.