|Project Title||Gene validation for high-value agronomic QTLs in rice|
|Project Type||Contained Use (Greenhouse)|
|Name of Institution||International Rice Research Institute|
|Supervising IBC||International Rice Research Institute (IRRI)|
Dr. John Platten
|Experimental Facility/Site||International Rice Research Institute (Genetics and Genomics Laboratory (GGL), Gene Transformation Laboratory (GTL), CL4).|
|Purpose / Objectives||
To validate candidate genes identified from whole-genome sequencing analyses as potentially controlling QTLs of agronomic importance. Traits under investigation are: Salinity tolerance Drought tolerance Anaerobic germination tolerance Seedling vigor Grain nutrient content Submergence tolerance Stagnant flooding tolerance Chalkiness Krantz anatomy Iron toxicity tolerance Aluminium toxicity tolerance
All work is to be conducted inside approved biosafety facilities (GGL, GTL, CL4) proof against typhoon, earthquake and flood. Seeds are to be stored in approved facilities (CL4) proof against typhoon, flood and earthquake. Materials to be disposed of by heat sterilization (autoclaving) and/or deep burial as per DOST-BC approved guidelines and under due supervision of the BPI-PEQS and the IRRI-IBC. Institutional contingency plans for accidental release are in place.
|Conditions for Approval||
a) All activities shall be conducted at the Genetics and Genomics Laboratory, Genetic Transformation Laboratory and CL4 Transgenic Laboratory and Glasshouse of the International Rice Research Institute (lRRl) in Los Baños, Laguna.
|Date of Approval (DD-MM-YYYY)||16-08-2014|
|Date of Completion (DD-MM-YYYY)||N/A|
Significant progress has been made worldwide in identifying regions of the rice genome that confer traits needed in plant breeding efforts. These include such traits as tolerance to abiotic stresses (salinity, drought, anaerobic germination, submergence, stagnant flooding, iron toxicity, aluminium toxicity), grain quality (grain nutrient content, chalkiness) and anatomical features (Krantz anatomy). However despite the success in identifying genomic regions controlling these, the translation of this success into breeding gains has been limited, due largely to the difficulties in utilizing large genomic regions in breeding processes. To overcome this it is necessary to identify the specific gene and mutation that confers the observed trait, from which markers diagnostic for the trait can then be designed.
The purpose of this project is to evaluate candidate genes from these genomic regions to identify which is controlling the trait, thus providing the information needed for diagnostic marker design. Candidate genes from donor and recipient rice varieties (Oryza sativa L.) will be transformed back into appropriate recipient rice varieties (Oryza sativa L.) to determine their effects on the trait of interest. Cloning, transformation and evaluation of lines will all occur in approved laboratory and glasshouse facilities at IRRI (Genetics and Genomics Laboratory, Genetic Transformation Laboratory, CL4 glasshouse facility). No field work is to be conducted.
Upon successful identification of a causal gene/mutation, this information will be used to design diagnostic markers for use in conventional breeding efforts. No transgenic products are produced; these are only required for trait evaluation - to confirm the involvement of specific genes - not for breeding. As there is large and ongoing need for this work, the initial phase of the project will stretch over 5 years.