|Project Title||Confined Field Trial of Iron Biofortified Transgenic Rice (Part 3)|
|Project Type||Contained Use (Laboratory)|
|Name of Institution||International Rice Research Institute|
|Supervising IBC||International Rice Research Institute (IRRI)|
Dr. Inez H. Slamet-Loedin
|Experimental Facility/Site||Blocks F1-F2, IRRI Experimental Station, IRRI, Los Banos, Laguna, Philippines|
|Purpose / Objectives||
The purpose of the confined trial is to evaluate promising transgenic lines that contain foreign genes that store and enhance the transport of iron in the grain. The confined field test is vital to evaluate high-iron containing lines as compared to ordinary rice. Previous conventional breeding efforts resulted to only 6-8 ppm (parts per million) in the grain, falling short of the targeted level of 13 ppm or even higher to make a significant health impact based on rice-based diet. Again, such objectives can be only achieved with the help of genetic engineering. Rice lines that have iron, based on laboratory analysis, will be tested in what is called the Lead Events Nursery (LEN) and Observation Nursery (ON). LEN requires more seeds planted in four identical replicates; ON, on the other hand, has lesser number of seeds planted with only two replicates. Two promising rice plants that had high iron content from the two previous confined trials at lRRl and one at CIAT will also be planted in an area that is allocated for 'seed increase' or for further propagation of the seeds for future studies.
|Conditions for Approval||
a) Before commencing with the activities, the DOST-BC and the Post Entry Quarantine Station, Bureau of Plant Industry (PEQS-BPI) shall inspect and approve the proposed site for the confined test (CT) located at Block F1-2 of the IRRI Experimental Station, Los Baños, Laguna
|Date of Approval (DD-MM-YYYY)||16-08-2014|
|Date of Completion (DD-MM-YYYY)||29-10-2015|
Biofortification of rice to increase micronutirent, particularly iron, is considered one of the options in helping solve the problem of micronutrient deficiency affecting a large percentage of the human population. Rice grain contains lower level of iron when milled. Increasing the iron content of the endosperm using iron storage protein and iron transporters could eventually introduce more iron into the grain. We have developed transgenic lines harboring iron storage protein gene, ferritin (NCBP ref. no. 1999-0119) and/or iron transporter genes in rice cultivar IR64 and high iron breeding line IR69428 under the NCBP approved project (NCBP ref no 2007-0221). These evenets have been evaluated in the transgenic screen house and increase in the iron content of several promising events was observed. This proposed confined field trial serves as a confirmation test of the performance of our two best lead events (1-IR64-IRS495-234 and 1-IR64-IRS495-27) selected from 12 transgenic events tested in the previous iron biofortification confined field trials (DOST-BC2011-0264 and DOST-BC2013-027). These will also serve as the benchmark of yield, iron and zinc levels (iron level close to targeted value of 13 ppm Fe was obtained in these events with a bonus of high Zn content). These two events will be included in the LEN (Lead event nursery) with other promising events (shown to have high Fe by ICP analysis and Prussion brlue staining) from other constructs. Testing of other promising events is needed to ensure that the best material will come out of the pipeline for the targeted goal of releasing high-iron materials in the farmer's field. A total 17 entries which include transgenic events, nulls and controls (wild type and tissue culture will be planted in four replications in the LEN).
The Observation Nursery (ON) will be composed of 58 entries, including transgenic events, nulls and controls planted in two replications. Selection of entries for the ON is based on intensity of grain iron staining using Perl's Prussian blue but have limited amount of seeds. These events are derived from five constructs in the varietal background of IR64 or IR69428. In addition, conventionally-bred materials obtained from the crossing of event 1-IR64-IR495-234 and 1-IR64-IRS495-274 with other popular varieties will be included in observation nursery, including heterozygous and homozygous lines of 1-IR63-IRS495-234 and 1-IR64-IRS495-274 crossed with IR 64 to clean up the tissue culture effect. In all plotes corresponding nulls and controls will also be planted. A portion of the confiend trial site will also be devoted for seed increase of 1-IR64-IRS495-234, 1-IR64-IR495-274 and IR64 wild type to produce sufficient seeds for further research as was done in DOST-BC-2013-0276.
The sire of the confined field test is Blocks F1 & F2, IRRI Experimental Station, Los Baños, Laguna.
The duration of the field trials is from 01 August 2014 to 28 February 2015.
The overall objective is basically the same as the two previous confined test, i.e., to select transgenic event with high dietary Fe and Zn in polished rice, and desirable agronomic performance, suitab le for future multi location trials.
The specific objectives are:
Public Information Sheet
|Title||Date of Approval||Date of Posting|
|Confined Field Trial of Iron Biofortified Transgenic Rice (Part 3)||08-22-2014||N/A|