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Project Information


Reference Number 20120267
Project Title Cloning and expression of the Plasmodium falciparum MSP-1 block 2 gene and biochemical characterization of its gene product
Project Type Contained Use (Laboratory)
Status Ongoing
Name of Institution University of the Philippines - Diliman
Cooperating Institution None
Supervising IBC University of the Philippines-Diliman (UPD)
Project Leader(s)

Dr. Denise Mirano-Bascos

Experimental Facility/Site Rm 308 - Central Molecular Biology Research Laboratory, 3/F National Institute of Molecular Biology and Biotechnology Building, National Science Complex, University of the Philippines, Diliman, Quezon City
Purpose / Objectives

General Objective of the program: To clone and express the MSP-1 block 2 gene of P. falciparum in bacteria in order to understand its role in the pathogenesis of the parasite. Specific objectives for this phase of the project (estimated time: 2 years)

1. To clone the MSP-1 block 2 gene of P. falciparum

2. To express the MSP-1 block 2 gene in bacteria

3. To characterize the gene product obtained

Biosafety measures

BSL-2 laboratory

Conditions for Approval

a) All activities shall be conducted at the Central Molecular Biology Research Laboratory, 3/F National Institute of Molecular Biology and Biotechnology Building, National Science Complex, University of the Philippines, Diliman, Quezon City

b) The proponent shall adhere as closely as possible to the schedule of activities reflected in the submitted Gantt chart

c) Any modifications in the schedule of activities shall be made with the concurrence of the UP Diliman-IBC and the DOST-BC

d) A biosafety contingency plan shall be submitted before the conduct of the experiment

e) The proponent shall be informed which activities would require the presence of the UP Diliman-IBC, DOST-BC and PEQS-BPI personnel

f) The proponent shall ensure that only the DOST-BC authorized personnel are allowed inside the experimental facilities

g) The DOST-BC and PEQS-BPI personnel should be informed in advance of any visitations by unauthorized persons

h) The DOST-BC and the PEQS-BPI personnel shall be informed immediately of any intrusion by unauthorized persons

i) The proponent shall ensure that stray animals are excluded from the experimental facilities while tests are being conducted

j) In case of undue destruction of experimental materials resulting from unauthorized entry of personnel or breach of containment of experimental facilities, the proponent shall implement measures to prevent the inadvertent escape of any viable material within the site

k) The proponent and the supervising IBC shall be held accountable for the undue destruction of the experimental materials and the consequences that their inadvertent escape may cause to the surrounding environment

l) All plants and viable materials within the experiment area shall be accounted for

m) The proponent shall strictly observe proper disposal procedures for all materials

n) Movement of all materials will be done in compliance with all relevant biosafety and phytosanitary requirements of the Philippines

o) Any additional requirement that the DOST-BC may impose, as necessary, during the duration of the experiment shall be complied with

p) The proponent shall submit through the IBC a completion report within 90 days after completion of the experiment.

Date of Approval (DD-MM-YYYY) 18-02-2012
Date of Completion (DD-MM-YYYY) N/A
Executive Summary N/A