Project Information

Reference Number 20120266
Project Title Confined field trial on evaluation of lowland transgenic lines for drought tolerance III
Project Type Contained Use (Laboratory)
Status Completed
Name of Institution International Rice Research Institute
Cooperating Institution Japan International Research Center for Agricultural Sciences (JIRCAS)
Supervising IBC International Rice Research Institute (IRRI)
Project Leader(s)

Dr. Inez Hortense Slamet- Loedin
Dr. Amelia Henry
Dr. Abdebagi Ismail

Experimental Facility/Site IRRI Experimental Station, confined field site (Block D20-23) Los Baños, Laguna
Purpose / Objectives

1. To evaluate the agronomic performance of IR64 transgenic lines selected from CFT in 2010DS and CFT2011DS in drought stress in lowland-drought field conditions

2. To evaluate agronomic performance of additional IR64 and MTU1010 promising lines selected based on the screenhouse performance.

Biosafety measures

The site is inside IRRI experimental station which will be supervised solely by authorized personnel/staff during the entire season of the trial. The entire harvested transgenic seeds will be packed in hermetically sealed aluminum foil packages and stored in locked refrigerators dedicated to storage of transgenic seeds only located in Rice Biotechnology Laboratory, PBGB division IRRI. All the rice straws/stubbles will be ploughed under the trial site. The trial site will be left follow for 30-45 days after harvest and volunteer plants will be ploughed under the trial site. The field trial will be accessed only by the authorized staff only who are directly involved in the experiment. Only the IRRI-approved visitors could access the site. (see table 4 for the list of authorized staff) Plant material movements will strictly follow the relevant biosafety and phytosanitary requirements of the Philippine Government.

Conditions for Approval

Before commencing with the activities, the DOST-BC and the Post Entry Quarantine Station, Bureau of Plant Industry (PEQS-BPI) shall inspect and approve the proposed site for the confined test (CT) located at Block 020-23 of the IRRI Experimental Station, Los Banos, Laguna. Prior to the initiation of the activities, the proponent/s shall discuss the monitoring schedule with the DOST-BC and the PEQS-BPI to identify the activities that will require their presence. The proponent/s shall furnish the DOST-BC and the PEQS-BPI with a copy of the agreed monitoring schedule. The DOST-BC approved Project Information Sheet (PIS) shall be posted for three (3) consecutive weeks in at least three (3) conspicuous places in the barangay and/or city/municipal halls where the proposed activities will be conducted. The PIS should be posted at least 15 days the actual transplanting of seeded transgenic rice in the confined test site. Proof of posting of the PIS, which may either be a certification from the duly authorized barangay leader(s) or an affidavit executed by the Proponent, should be submitted to the DOST-BC within ten (10) days from the last day of posting. The proponent shall adhere as closely as possible to the schedule of implementation and monitoring activities for this trial. Modifications in the planting schedule and other activities may be made only with the concurrence of the IBC and DOST-BC. At least 150m physical or temporal reproductive isolation of the GMO shall be observed at all times. The proponent must ensure that the flowering stage of the transgenic drought-tolerant rice plants shall not coincide with the flowering of the other rice plants adjacent to or within the confined test site. All confinement measures should be in place before the actual transplanting of rice seedlings. The proponent shall ensure that only authorized personnel are allowed inside the test site. The DOST-BC and the PEQS-BPI shall be informed in advance of any visitations by unauthorized personnel. The DOST-BC and the PEQS-BPI shall be informed immediately of any intrusion by unauthorized persons. The proponent shall ensure that stray animals are excluded from the test site while trials are being conducted. The proponent shall ensure the security of the trial, the field workers and the monitors. In case of undue destruction of the experimental materials resulting from unauthorized entry of personnel or breach of containment of the CT facility, the proponent must implement measures to prevent the inadvertent escape of any viable material within the site. The proponent and the supervising IBC shall be held accountable for the undue destruction of the experimental materials and the consequences that its inadvertent escape may cause to the surrounding environment. The rice grains or any plant parts shall not be eaten or fed to humans, wildlife and livestock. The proponent shall provide a space within the test site for disposal of the used plant materials. The harvested grains shall be properly labeled and stored at the designated Seed Storage Facility where transgenic seeds are kept, while plant materials and other plant debris used within the test site should be buried in a pit within the test site or plowed under. The proponent shall leave the test site fallow for at least 30 days. During the fallow period, the proponent shall strictly monitor emergence of volunteer plants and plant pests/diseases within the site for two (2) consecutive weeks after harvest. Furthermore, the proponent shall coordinate with the PEQS-BPI personnel during this period for the monitoring of the fallow site. If necessary, the proponent may irrigate the test site to induce germination of seeds. Any volunteer plants that will emerge, or any ratoon residues shall be uprooted and disposed either by plowing under or by burying in the pit intended for transgenic plant biomass from this confined field test. The proponent shall comply with additional requirements that the DOST-BC may impose, as necessary, during the experiment period. The proponent shall submit through the IBC a completion report 90 days after the official completion of the experiment which shall be determined by the PEQS-Bpi monitors after completion of all post-harvest monitoring of volunteers.

Date of Approval (DD-MM-YYYY) 21-01-2012
Date of Completion (DD-MM-YYYY) 05-06-2012
Executive Summary N/A


Public Information Sheet

Title Date of Approval Date of Posting
Confined field trial on evaluation of lowland transgenic lines for drought tolerance III 02-02-2012 N/A