Project Information

Reference Number 2022-0341
Project Title Genetically Engineered E. coli with Lignin-Degrading Activity Towards Future Bioethanol Production from Rice Straw
Project Type Contained Use (Laboratory)
Status Ongoing
Name of Institution Philippine Rice Research Institute
Cooperating Institution N/A
Supervising IBC Philippine Rice Research Institute CES (PhilRice) - Muñoz, Nueva Ecija
Project Leader(s)

Reynante L. Ordonio, Ph.D.

Experimental Facility/Site CBC-GTL, PhilRice-CES, Brgy. Maligaya, Science City of Munoz, Nueva Ecija
Purpose / Objectives

Generally, the objective of this study is to genetically engineer
bacteria with lignin-degrading activity.
Specifically, it aims to:

1. Combine the Trametes versicolor Lip1 gene and
bacteriophage T7 terminator in a vector plasmid (pUC19) and
transform it into E. coli.

2. Select transformed bacteria with lignin-degrading activity.

Biosafety measures

The Genetic Transformation Laboratory is housed in the
Laboratory Wing of the new Crop Biotechnology Center facility
within the PhilRice-CES compound

Living modified organisms (E coli) will be used in the BL2
laboratory under aseptic conditions by trained personnel. Any
implements that come into contact with them will be autoclaved
and disposed properly. Living tissues will be autoclaved before
they are discarded.

Access to the laboratory will be given to project staff and
authorized personnel and to DOST-BC-approved visitors. Only
one entry point will be provided, which will be secured at all times.
Movement of people within the area will also be documented. A
logbook will be maintained to record the entry of personnel and
their activities.

Movement of all materials will be done in compliance with all the
relevant biosafety and phytosanitary requirements of the
Philippine Government.

Any unplanned activity will be monitored and reported. There will
be a logbook and other monitoring systems. Accidental release
will be taken care of by cleaning the contaminated area. A
thorough investigation of accidental release will be conducted to
prevent similar incidents from happening in the future. Any case
of sabotage will be immediately reported to the appropriate

Conditions for Approval N/A
Date of Approval (DD-MM-YYYY) N/A
Date of Completion (DD-MM-YYYY) N/A
Executive Summary N/A