Reynante L. Ordonio, Ph.D.

Generally, the objective of this study is to genetically engineer
bacteria with lignin-degrading activity.
Specifically, it aims to:

1. Combine the Trametes versicolor Lip1 gene and
bacteriophage T7 terminator in a vector plasmid (pUC19) and
transform it into E. coli.

2. Select transformed bacteria with lignin-degrading activity.

The Genetic Transformation Laboratory is housed in the
Laboratory Wing of the new Crop Biotechnology Center facility
within the PhilRice-CES compound

Living modified organisms (E coli) will be used in the BL2
laboratory under aseptic conditions by trained personnel. Any
implements that come into contact with them will be autoclaved
and disposed properly. Living tissues will be autoclaved before
they are discarded.

Access to the laboratory will be given to project staff and
authorized personnel and to DOST-BC-approved visitors. Only
one entry point will be provided, which will be secured at all times.
Movement of people within the area will also be documented. A
logbook will be maintained to record the entry of personnel and
their activities.

Movement of all materials will be done in compliance with all the
relevant biosafety and phytosanitary requirements of the
Philippine Government.

Any unplanned activity will be monitored and reported. There will
be a logbook and other monitoring systems. Accidental release
will be taken care of by cleaning the contaminated area. A
thorough investigation of accidental release will be conducted to
prevent similar incidents from happening in the future. Any case
of sabotage will be immediately reported to the appropriate
authorities.