Dr. Sung-Ryul Kim
Dr. Nese Sreenivasulu
Dr. Inez H. Slamet-Loedin

To study molecular mechanism of the long-exserted stigma gene, promoter analysis using GFP and GUS reporter genes, promoter-swapping, and promoter modification experiments will be conducted. In addition, allele replacement of the long-exserted stigma gene will be tried in commercial hybrid parental backgrounds to see the potential values of the GEd products for commercialization in the future.

Appropriate biosafety measures as implemented by IRRI, and the
requirements of the DOST-BC will be strictly observed and
followed. Transgenic and untransformed control plants will only
be grown in CL4 glass bays, a well-contained facility.

Biosafety measures that will be taken:

• 
  All vectors will be kept in the plasmid collection of Genetic
  Transformation Laboratory (GTL) after construction.
• The tested plants will be kept in isolation in the CL4 glass
  bays to prevent access of unauthorized personnel.
• Plant materials for disposal will be placed in sealed plastic
  bags and disposed of in dedicated disposal pit for
  transgenic materials.
• Plants grown in CL4 will be destroyed and the pots and
  soil will be autoclaved at high temperature and high
  pressure.
• All materials movement and disposal will be done
  following standard internal protocol and SOPs
• Access to GTL, GIV and CL4 facilities are for authorized
  staff only.
• A barcode entry system is applied in the facilities to
  record the entry of personnel; a log book is available for
  visitors not having IRRI access card system.

Transgenic plants from CL4 will be destroyed after the completion
of the experiment. The seeds from transgenic plants during the conduct of the study will be kept at CL4 seed storage room. And after completion of the project, seeds will be kept for additional 3 years for publication purpose as an evidence of gene validation tests.

Handling of transgenic materials like movement and disposal and
other necessary biosafety measures will be observed and
implemented following guidelines set by IRRI’s Biosafety Office
and IRRI’s Stewardship Implementation Team.

Researchers that will be working on the transgenic materials are
required to attend training and orientation on the proper handling
of transgenic materials and will need to pass the Good Laboratory
Practice (GLP) Course. IRRI also implements stewardship
practices that meet Excellence Through Stewardship standard
meant to complement Biosafety.