Project Information

Reference Number 2021-0336
Project Title Improving popular released rice varieties through gene editing
Project Type Contained Use (Laboratory)
Status Ongoing
Name of Institution Philippine Rice Research Institute
Cooperating Institution N/A
Supervising IBC Philippine Rice Research Institute CES (PhilRice) - Mu├▒oz, Nueva Ecija
Project Leader(s)

Reynante L. Ordonio, PhD.

Experimental Facility/Site Biosafety Level 2 (BL2) Screenhouse and laboratory, Philippine Rice Research Institute, Brgy. Maligaya, Muñoz, Nueva Ecija, Philippines
Purpose / Objectives

Generally, our objective is to produce improved lines of popular
released varieties that carry a combination of useful traits
through CRISPR-CAS technology.
Specifically, we aim to:

1. Identify and assemble suitable recipient popular indica
varieties that respond well to tissue culture;

2. To design appropriate constructs for gene editing by
CRISPR-Cas9 technology involving the following traits: rice
tungro spherical virus (RTSV) resistance, bacterial leaf blight
(BLB) resistance, lower amylose content than the original (if
needed) and aroma; and

3. To screen for successfully edited plants by DNA
sequencing, expression analysis, phenotyping and PCR

Biosafety measures

The BL2 Screenhouse is within the premises and under total
control of PhilRice-CES.
Living modified organisms (E coli and Agrobacterium) will be used
in the BL2 laboratory under aseptic conditions by trained
personnel. Any implements that come into contact with them will
be autoclaved and disposed properly. The use of floral dip will also
be done in the laboratory under aseptic conditions, after which,
the plants will be transported back to the BL2 screenhouse under
IBC monitoring.

Access to the BL2 screenhouse will be given to project staff and
authorized personnel and to DOST-BC-approved visitors. Only
one entry point will be provided, which will be secured at all times.
Movement of people within the area will also be documented. A
logbook will be maintained in the CL2 screenhouse to record the
entry of personnel and their activities. Moreover, the CL2
screenhouse facility will be inspected regularly to ensure
adequate protection against rats, birds, etc.

Gene-edited seeds harvested from the BL2 screenhouse will be
kept in sealed/locked containers separate from other ordinary
materials. Movement of all materials will be done in compliance
with all the relevant biosafety and phytosanitary requirements of
the Philippine Government.

Collection and transport of plant materials from the BL2
screenhouse to the BL2 Isolation Room will be supervised by an
IBC representative. Access to the Isolation Room will be restricted
to authorized personnel only. Living tissues will be autoclaved
before they are discarded. Transport of seedling regenerants from
the BL2 laboratory to the BL2 screen house will also be implemented under IBC supervision.

Plant materials for disposal will be placed in sealed plastic bags.
CL2-grown plants for disposal will be autoclaved and then put in
the concrete compost bin near the area.

Any unplanned activity will be monitored and reported. There will
be a logbook and other monitoring systems. Accidental release
will be taken care of by cleaning the contaminated area. A
thorough investigation of accidental release will be conducted to
prevent similar incident from happening in the future. In the
extreme event of an accident arising at the field site, all plant
materials in the experimental plots will be plowed back into the
soil. Any case of sabotage will be immediately reported to the
appropriate authorities.

Conditions for Approval N/A
Date of Approval (DD-MM-YYYY) N/A
Date of Completion (DD-MM-YYYY) N/A
Executive Summary N/A