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Project Information


Reference Number 20180317
Project Title Contained Use of New Yeast in Pilot Trial (Demonstration Test) for Cellulosic Ethanol Production Technology in the Philippines
Project Type Contained Use (Laboratory)
Status Ongoing
Name of Institution Roxas Holdings, Inc. / Nippon Steel & Sumikin Engineering CO., LTD.
Cooperating Institution Roxol Bioenergy Corporation
Supervising IBC Roxas Holdings Inc.
Project Leader(s)

Mr. Takeshi Nishi

Experimental Facility/Site Plant Site of Central Azucarera Don Pedro, Inc. Brgy. Lumbangan, Nasugbu, Batangas
Purpose / Objectives

The activity is for R&D purpose for establishment of the new technology, cellulosic ethanol production technology. The technology produce ethanol from cellulosic biomass such as sugarcane bagasse, agricultural residue, etc. which the Philippines has a lot.

The pilot trial is currently running under permission of "DOST-BC Ref. NO. 2015-0296: Pilot Trial (Demonstration Test) for cellulosic ethanol technology in the Philippines".

The purpose of this application is to introduce the new genetically modified yeast, UzEt17, to the pilot trial in the Philippines in addition to original yeast.

 

Biosafety measures

Genetically modified yeast (GMY) is to be used in ethanol fermentation process for a high ethanol yield in the pilot trial. The host yeast of the GMY is a wine yeast and has been used in wine distilleries for a long time in Japan. And foreign genes for the GMY are derived from microorganisms recognized as safe. Thus the GMY is officially declared that there is no risk of illness or harm in human.

In the pilot trial, there are 2 places handling the GMY. One is the laboratory for culture of the yeast and analysis of culture fluids, and the other is the fermentation facility (culture tank, fermentation tank etc.) of the pilot plant. The laboratory and the pilot plant are contiguous. Since the GMY is considered to be safe, the facilities for the yeast are designed in compliance with Biosafety Level 1 (BL1 or BL1-LS) of physical containment defined in "The Philippines Biosafety Guideline for Contained Use of Genetically Modified Organisms (GMOs)".

Conditions for Approval

a) All activities shall be conducted at the Plant Site of Central Azucarera Don Pedro, lnc. in Brgy Lumbangan, Nasugbu, Batangas;

b) The proponent shall submit to the DOST-BC a modified Gantt chart and shall adhere as close as possible to the revised schedule of activities;

c) Any modifications in the schedule and additional activities must be communicated to the DOSTBC for information and/or approval;

d) All confinement measures should be in place prior to the commencement of the activities;

e) Signage should be in place indicating that the facility is a restricted/limited access facility and only those personnel approved by the DOST-BC maybe allowed access;

f) Only authorized personnel as indicated in the proposal shall be allowed access to the facility for the entire duration of the experiment;

g) Monitoring for biosafety compliance shall be conducted by the lnstitutional Biosafety Committee (lBC), report of which shall be submitted to the DOST-BC within five (5) working days after the conduct of the monitoring activity;

h) Measures to limit access of unauthorized persons should be strictly observed during conduct of the experiment and that the designated facility must be kept secured at all times;

i) The DOST-BC shall be informed in advance of any visitations by unauthorized personnel for approval.

j) The DOST-BC shall be informed immediately of any intrusion by unauthorized persons;

k) The proponent shall ensure that stray animals are excluded from the facilities at all times;

l) ln case of incident or undue destruction in the facility resulting from unauthorized entry of personnel or breach in containment, the proponent shall implement the biosafety contingency measures to prevent the inadvertent escape of any viable materials to the outside environment;

m) The proponent and the supervising IBC shall be held accountable forthe undue destruction of the experimental materials and the consequences that their inadvertent  escape may cause to the surrounding environment; and

n) All viable materials within the experiment area shall be duly accounted for.

Date of Approval (DD-MM-YYYY) 24-03-2018
Date of Completion (DD-MM-YYYY) N/A
Executive Summary

This activity is for R&D purpose pursuing establishment of the new technology of cellulosic ethanol production. The technology can produce ethanol from cellulosic biomass such as sugarcane bagasse, agricultural residue, napier grass, etc. which the Philippines has a lot.

The purpose of this project is to introduce the new genetically modified yeast, UzEt17, to the pilot trial in the Philippines in addition to original yeast, UzEt14. The pilot trial is currently running under permission of "DOST-BC Ref. No. 2015-0296: Pilot Trial (Demonstration Test) for cellulosic ethanol technology in the Philippines". The yeasts, UzEt14 and UzEt17, have been developed by Toyota Motor Corporation in Japan. The new yeast UzEt17 has higher performance of ethanol yield than original yeast UzEt14.

In the fermentation process, a genetically modified yeast is needed for a high ethanol yield from cellulosic biomass. The host yeast of UzEt17 is Saccharomyces cerevisiae OS-2 which was isolated before 1937 from winery or grape and has been supply as wine yeast and used in wine distillery for long time in Japan. The host yeast and the organisms of the foreign genes have been classified as a "generally recognized as safe (GRAS)" in a risk assessment report which was published by U.S. Environmental Protection Agency. Therefore UzEt17 is considered that there is no illness and harm to human as well as the original yeast UzEt14.

The outline of this project is as follows:

- Pilot plant capacity: Feedstock treatment capacity 2 wet-tons/day (1 dry-ton/day equivalent)
- Pilot plant process: Pretreatment, Saccharification, and Fermentation (Distillation process of the plant is to be omitted because it is an proven technology)
- Plant area: approx. 45m x 55m
- Feedstock: Sugarcane bagasse, Sugarcane residue in the farmland, and other potential cellulosic biomass
-Location: Plant site of Central Azucarera Don Pedro, Inc., Brgy. Lumbangan, Nasugbu, Batangas
-Project period: July, 2016 to February 2018
-Schedule for the new yeast UzEt17: Importation from Japan on February 2018, Removal from the pilot plant on February 2019

 

In the pilot trial, theres are 2 places handling UzEt17. One is a laboratory for culture of the yeast and analysis of culture fluids. The other is the fermentation facility (culture tank, fermentation tank etc.) of the pilot plant. The laboratory and the pilot plant are contiguous. Since UzEt17 is considered as safe, the facilities for the yeast are designed in compliance with Biosafety Level 1 (BL1 or BL1-LS) of physical containment defined in "The Philippines Biosafety Guideline for Contained Use of Genetically Modified Organisms (GMOs)".