|Project Title||2018 lRRl Confined test of lron and Zinc biofortified transgenic rice|
|Project Type||Confined Test|
|Name of Institution||International Rice Research Institute|
|Supervising IBC||International Rice Research Institute (IRRI)|
lnez H. Slamet-Loedin
|Purpose / Objectives||
The overall objective is to select three to four lead transgenic events with high dietary Fe and Zn (HIZR) in polished rice, with desirable agronomic performance, suitable for future multilocation trials.
|Conditions for Approval||
a) Activities shall be conducted at the HIZR confined test area inside the Transgenic Research Facility (TRF) of Zeigler Experimental Station (ZES) at the International Rice Research Institute (IRRI), Los Banos, Laguna.
b) Prior to the initiation of the activities, the proponents shall discuss the monitoring schedule with the Department of Agriculture-Bureau of Plant Industry-National Plant Quarantine Service Division (DABPl-NPQSD) to identify the activities that will require their presence. The proponents shall furnish the DOST-BC with a copy of the agreed monitoring schedule .
c) Monitoring for biosafety compliance during stages of the project implementation, such as, but not limited to, the movement of transgenic materials, transplanting, reproductive stage, harvest, postharvest monitoring and termination, shall be conducted by the IBC. Report of which shall be submitted to the DOST-BC not more than five (5) days after the conduct of the monitoring activity.
d) The DOST-BC approved Project Information Sheet (PIS) shall be posted for three (3) consecutive weeks in the public announcement board of the barangay(s) and city or municipality where the proposed activities will be conducted.
e) Proof of posting of the PIS, which may either be a certification from the duly authorized Barangay leader(s) or an affidavit executed by the Proponent , should be submitted to the DOST-BC within ten (10) days from the last day of posting.
f) A letter will be issued to the IBC informing that planting/transplanting on the confined test site can commence.
g) The proponent shall adhere as closely as possible to the schedule of implementation and monitoring activities for this activity.
h) Modifications in the planting schedule and other activities must be communicated to the DOST-BC for information and/or approval.
i) Movement of all transgenic materials should be done in compliance with the DOST-BC and the DA-BPl-NPQSD guidelines.
j) At least 150 meters physical or temporal reproductive isolation of the GMO shall be observed at all times.
k) All confinement measures should be in place before the actual transplanting of rice seedlings.
l) The proponent shall ensure that only authorized personnel are allowed inside the test site.
m) The DOST-BC shall be informed in advance of any visitations by unauthorized personnel for approval.
n) The DOST-BC shall be informed immediately of any intrusion by unauthorized persons.
o) The proponent shall ensure that rodent control measures are in place and that stray animals are excluded from the test site while trials are being conducted.
p) The proponent shall ensure the security of the trial, the field workers, and the monitors.
q) In case of undue destruction of the experimental materials resulting from unauthorized entry of personnel or breach of containment of the confined test facility, the proponent should implement measures to prevent the inadvertent escape of any viable material within the site.
r) The proponent and the supervising IBC shall be held accountable for the undue destruction of the experimental materials and the consequences that its inadvertent escape may cause to the surrounding environment.
s) The rice grains or any plant part shall not be eaten or fed to humans, wildlife and livestock.
t) The proponent shall provide a space within the test site for disposal of the used plant materials.
u) The harvested grains shall be properly labeled and stored at the designated seed storage facility where transgenic seeds are kept, while plant materials and other plant debris used within the test site should be buried in a pit within the test site or plowed under.
v) The proponent shall leave the test site fallow for at least 30 days. During the fallow period, the proponent shall strictly monitor emergence of volunteer plants and plant pests/diseases within the site for two (2) consecutive weeks after harvest. If necessary, the proponent may irrigate the test site to induce germination of seeds. Any volunteer plants that will emerge, or any ratoon residues shall be uprooted and disposed either by plowing under or by burying in the pit intended for transgenic plant biomass from this confined field test.
w) The IBC shall determine whether the confined test site shall be released from post-harvest restrictions. This shall form part of the collated post-harvest monitoring report of the project to be submitted IBC.
x) The proponent shall comply with additional requirements that the DOST-BC may impose, as necessary, during the experiment period.
y) The proponent shall submit through the IBC a completion report ninety (90) days after the official completion of the confined test after completion of all post-harvest monitoring of volunteers.
|Date of Approval (DD-MM-YYYY)||24-03-2018|
|Date of Completion (DD-MM-YYYY)||N/A|
lron (Fe) andZinc (Zn) deficiencies are the most common micronutrient deficiencies globally affecting more than 2 billion people. Biofortification of rice to increase micronutrient content, particularly Fe and
Rice grains of commonly cultivated varieties contain low levels of Fe when milled, while in brown rice, Fe and Zn in the bran are mostly bound to phytate, hence they are not available for absorption by the
We developed transgenic indica rice lines (using cultivars lR64 and 1R69428 as backgrounds) harboring iron storage protein ferritin gene from soybean (NCBP Ref. No. 1999-01 19) and iron
This proposed confined test aims to evaluate and select three to four candidate lead events (from eight independent events) with high dietary Fe and Zn (HIZR) in polished rice grains and with
The site of the confined test is the confined test area designated for High lron and Zinc rice inside the Transgenic Research Facility (TRF) of Zeigler Experimental Station (ZES) at the lnternational Rice