Project Information

Reference Number 20170312
Project Title Functional validation of selected candidate genes and promoters involved in increasing yield potential in rice cultivars
Project Type Contained Use (Laboratory)
Status Ongoing
Name of Institution International Rice Research Institute
Cooperating Institution N/A
Supervising IBC International Rice Research Institute (IRRI)
Project Leader(s)

Dr. Inez H. Slamet-Loedin
Dr. Ajay Kohli

Experimental Facility/Site Genetic Transformation Laboratory (GTL), Genetics and Genomics Laboratory (GGL), Transgenic plants are grown under controlled conditions in CL4 greenhouse (KJ Lampe Laboratory), approved facility for transgenic plant growth with standard measures for pest and disease control and in the screenhouses TG-01 A&B
Purpose / Objectives

To validate gene function of various candidate genes involved in different yield components in rice which can be used for further advanced breeding.

Biosafety measures

Appropriate biosafety and phytosanitary import regulations will be followed. Transgenic and untransformed control plants will be grown in CL4 greenhouses and biosafety screenhouse.

Biosafety measures that will be taken:
- All vectors will be kept in the plasmid collection of Genetic transformation lab, GB, IRRI Los Baños after construction.
- The tested plants will be kept in isolation in the CL4 transgenic greenhouse to prevent access of unauthorized personnel.
- Plant materials for disposal will be placed in sealed plastic bags.
- Plants grown in CL 4 will be destroyed and the pots and soil will be autoclaved at high temperature and high pressure.
- Plant materials that will be grown and evaluated in biosafety screenhouse will be disposed through appropriate disposal system in compliance with biosafety and related regulations in the Philippines.
- Access to screenhouse is for authorized staff only.
- A barcode entry system is applied in the screenhouse to record the entry of personnel; a log book is available for visitors not having IRRI access card system.
- Screenhouse facilities are adequate to prevent entry of rats and birds.
- Transgenic seeds harvested from CL4 and the screenhouse will be kept in hermetically-sealed aluminum foil packages and stored in locked refrigerators designated for storage of transgenic seeds in Rice Biotechnology Laboratory, IRRI.
- Movement of all seeds and plant materials will be done in compliance of all relevant biosafety and phytosanitary requirements of the Philippines.

Conditions for Approval N/A
Date of Approval (DD-MM-YYYY) 18-11-2017
Date of Completion (DD-MM-YYYY) N/A
Executive Summary

Rice (Oryza sativa L.) is the most important food crop in the world, feeding over half of the global population. In modern rice farming, high yield has accordingly become one of the major objectives of
breeders and growers over recent decades (Wang and Li, 2008; Xing and Zhang, 2010). Yield potential of rice has fallen significantly since 1990 even though rice yield has increased by green revolution technologies. Keeping in view on the increase in demand of rice and increasing population, we need to produce enough rice to feed the population by using less land, less water and less labor. It is predicted that there is a need to increase 26% more rice in the next 20 years to feed the population (IRRI, 2010). Rice production can be increased either by planting rice in additional land or increasing the productivity per unit area yield potential of rice varieties. Therefore, increasing rice yield in the
existing rice land must be the primary strategy to have higher rice production. Rice yield per plant is determined by three component traits: number of panicles per plant, number of grains per panicle, and grain weight (Wang and Li, 2008; Xing and Zhang, 2010). To date, a number of genes have been shown to influence these yield traits: Hybrid rice varieties have some strength for  higher production; but farmers hesitate to cultivate hybrid rice varieties because of high cost of F1 hybrid seeds. To enhance out-crossing rate to increase hybrid seed production, an increased size of stigma which is a part of pistil trait to capture pollen grains (male genetic materials) freely will enhanced seed production.

To validate the function of the potential candidate genes for yield potential and stigma elongation
1. Over expression study in commercial varieties like IR64, IR64, Nipponbare, Swarna, Ciherangand other backgrounds of the main candidate genes.
2. CRISPR/Cas9-induced target gene knock-out of candidate genes involved in high yield potential trait.
3. Endogenous promoter studies.
4. Alleles replacement of commercial relevant rice varieties with the corresponding alleles from landraces, wild rice and tropical japonica that has been proven to show improved agronomic trait will
allow development of high yielding rice. Initially the experiments will be conducted for proof-of-concept work and with a potential for an actual product in the future.

This Proposal duration is for 3 years.