|Project Title||Standardization of different genome editing systems and accelerated testing of C4 and photosynthetic candidate genes using genome editing and transient/stable expression in tobacco (transient only), algae and rice|
|Project Type||Contained Use (Laboratory)|
|Name of Institution||International Rice Research Institute|
|Supervising IBC||International Rice Research Institute (IRRI)|
Anindya Bandyopadhyay William Paul Quick
|Experimental Facility/Site||C4 Rice Center lab, C4 seed room, GTL, CL4, BG-15, BG- 16 and TG-01(c and d), International Rice Research Institute|
|Purpose / Objectives||
1. Develop genome editing tools for algae and rice
All the proposed work will be carried out strictly under the IRRI-IBC and DOST guidelines.
|Conditions for Approval||N/A|
|Date of Approval (DD-MM-YYYY)||18-11-2017|
|Date of Completion (DD-MM-YYYY)||N/A|
Precision genome engineering has been rapidly improved in recent years by development of targetable cleavage systems. Zinc-finger nucleases (ZFNs) and TALENs have already been deployed successfully for several crop species. However, the nonspecificity of the ZFNs and complex protein engineering process of TALENs keep them away from being the method of choice for validation of candidate genes. The recent discovery of CRISPR/Cas9, CRISPR/Cpf1, CRISPR/C2c2, CRISPR/Cas13b and allied systems offer a quick, easy and effective system for genome editing, particularly for knock out of gene function. The goal of this project can be summarized into two different sections. The first one will be to develop efficient genome editing tools for algae and rice and the second would be to apply the different developed genome editing tools in rice and algae for different traits like enhancement of photosynthetic efficiency or biomass accumulation. Construction of binary vectors and plant transformations as well as the molecular characterizations will be done following the standard protocol established in C4 Rice Center and Genetic Transformation Laboratory (GTL) at IRRI. The regenerated transgenic plants will be grown in contained glass house (CL4) or its equivalent screen houses that have passed the biosafety requirements. The sole aim of these experiments is to establish efficient tools of genome editing in plants which may further be used in future for enhancement or knocking down of different traits. The functional analysis of the edited genes will be mainly carried out in T1/T2 generation in rice. Plants with interesting phenotype can be further exploited as pre-breeding material to combine with other transgenes at IRRI and/or other collaborative institutes. The seeds generated from the project will be stored in C4 seed room.