|Project Title||Investigating the role of aquaporin genes in drought and salinity tolerance in rice (Oryza sativa L.)|
|Project Type||Contained Use (Greenhouse)|
|Name of Institution||International Rice Research Institute|
|Cooperating Institution||Supagro/University of Montpellier|
|Supervising IBC||International Rice Research Institute (IRRI)|
Dr. Amelia Henry
|Experimental Facility/Site||International Rice Research Institute Los Baños, Laguna, Philippines|
|Purpose / Objectives||
This study will be conducted to test transgenics for aquaporin constructs in the Nipponbare background under drought and salinity stress. Aquaporins are water channels that are expressed in plant membranes and are reported to be related to plant stress response. Since aquaporins are related to water transport, they may also control osmotic adjustment and would be good targets for improving understanding of plant responses to drought and salinity stress. The aquaporin genes that will be studied are known to be located in specific cell membranes (the plasma membrane and the tonoplast).
Experiments will be conducted in the approved transgenic screenhouse CS-02 at IRRI. Processing of samples will be confined in this approved transgenic facility. All transgenic rice seeds that will be generated from the experiments will be discarded accordingly. Seeds will be autoclaved before disposal and vegetative material will either be ploughed under or disposed of in designated disposal pits . No materials will be retained.
|Conditions for Approval||
a) Activities shall be conducted at the CS-02 transgenic screenhouse located at the International Rice Research Institute in Los Baños, Laguna.
b) Prior to the initiation of the activities, the proponent/s shall discuss the monitoring schedule with the Department of Agriculture-Bureau of Plant Industry (DA-BPI) to identify the activities that will require their presence. The proponent/s through the IBC shall furnish the DOST-BC with a copy of the agreed monitoring schedule.
c) The proponent shall adhere as closely as possible to the schedule of activities reflected in the submitted Gantt chart.
d) Any modifications in the schedule of activities shall be made with the concurrence of the IRRI-IBC and the DOST-BC.
e) Signage should be in place indicating that the facilities is restricted/limited access;
f) The proponent must provide a logbook to record access of personnel to the experimental facility.
g) The proponent must take measures to ensure that only the DOST-BC authorized personnel are allowed inside the experimental facilities.
h) The experimental facilities must be kept under lock when not in use.
i) In case of undue destruction of experimental materials resulting from unauthorized entry of personnel or breach of containment of experimental facilities, the proponent shall implement measures to prevent the inadvertent escape of any viable material within the facility.
j) The proponent and the supervising IBC shall be held accountable for the undue destruction of the experimental materials and the consequences that their inadvertent escape may cause to the surrounding environment.
k) The DOST-BC should be informed in advance of any visitations by unauthorized persons.
l) The DOST-BC shall be informed immediately of any intrusion by unauthorized persons.
m) The proponent shall strictly observe proper disposal procedures for all experimental materials.
n) Movement of all materials will be done in accordance with all relevant biosafety and phytosanitary requirements of the Philippines.
o) Any additional requirement that the DOST-BC may impose, as necessary, for the duration of the experiment shall be complied with.
p) The porponent shall submit through the IBC a completion report within 90 days after completion of the experiment following the prescribed format.
q) The IBC shall submit to the DOST-BC a report on the completion of this project following the prescribed format.
|Date of Approval (DD-MM-YYYY)||22-07-2017|
|Date of Completion (DD-MM-YYYY)||N/A|
Water channels, also termed aquaporins, play a crucial role in water transport in plants. Aquaporins may be related to controlling osmotic adjustment and would be good targets for improving understanding of plant responses to drought and salinity stress. The objective of this project is to test transgenics for aquaporin constructs in the Nipponbare background under drought and salinity stress conditions. The genes to be tested are components of key compartments en route towards to the vacuole. This role in the trafficking towards the vacuole may be essential for osmotic adjustment and for the optimal sequestration of Na+ in this compartment.
Seeds of Nipponbare, empty vector control line, wild type and 2 independent lines each of P35S::OsPIP1;1-GFP, P35S::OsPIP2;4-GFP, P35S::OsPIP2;5-GFP, P35S::OsTIP1;1-mCherry and P35S::OsTIP2;2-mCherry will be imported to IRRI from the University of Montpellier, France. No transformation will be conducted at IRRI. Two types of experiments will be conducted, drought screening which will be done under paddy conditions and salinity screening which will be a pot experiment. Both experiments will be conducted at the CS-02 screenhouse at IRRI. Physiological stress response attributes and grain yield will be measured. All materials will be destroyed after the study has been completed. The duration of the experiment will be one year.