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Project Information

Reference Number :
20160299
Project Title :
Introgression of 'Golden Rice' Event E into high-yielding rice varieties and disease resistant lines through marker assisted breeding
Project Type :
Contained Use (Screenhouse)
Status :
Ongoing
Name of Institution :`
Cooperating Institution:
International Rice Research Institute (IRRI)
Supervising IBC:
Philippine Rice Research Institute (PhilRice) - Muñoz, Nueva Ecija
Project Leader(s) :
Dr. Jonathan M. Niones
Dr. Reynante L. Ordonio
Dr. Roel R. Suralta
Experimental Facility/Site:
Containment Level 2 (CL2) Screen house, Philippine Rice Research Institute, Brgy. Maligaya, Muñoz, Nueva Ecija, Philippines
Purpose / Objectives :

1. To develop introgression lines with GR genes [phytoene synthase (psy) and carotene desaturase I (crtI) from the high-yielding commercial Philippine rice varieties and disease resistant rice line background through hybridization and marker-assisted selection

2. To fast-track the production of homozygous disease resistant lines through anther culture

3. To validate the resistance of selected lines by tungro and BLB screening 

4. To conduct marker-assisted selection to verify that phenotypic data correspond with genotype until stable lines possessing the traits of interest are developed

5. To select elite breeding lines with coparable levels of beta-carotene or better than the donor parent

Biosafety measures:

The CL2 Screen house is within an experimental station under total control of PhilRice-CES.

Access to the CL2 screen house will be given to project staff and authorized personnel and to DOST-BC-approved visitors. Only one entry point will be provided, which will be secured at all times. Movement of people within the area will also be documented. A logbook will be maintained in the CL2 screen house to record the entry of personnel and their activities. Moreover, the CL2 screen house facility will be inspected regularly to ensure adequate protection against rats, birds, etc.

Transgenic seeds harvested from the CL2 screen house will be kept in sealed/lock containers separate from other ordinary materials. Movement of all materials will be done in compliance with all the relevant biosafety and phytosanitary requirements of the Philippine Government.

Collection and transport of boot/spikelet samples for anther culture from the CL2 screen house to the CL2 Isolation Room will be supervised by an IBC representative. Access to the Isolation Room will be restricted to authorized personnel only. The culture vessels and their location in the culture rooms will be clearly marked. Living tissues will be autoclaved before they are discarded. Transport of seedling regenerants from the CL2 laboratory to the CL2 screen house will also be implemented under IBC supervision.

Plant materials for disposal will be placed in sealed plastic bags. CL2-grown plants for disposal will be autoclaved/incinerated and then put in the concrete compost bin near the area.

Any unplanned activity will be monitored and reported. There will be a logbook and other monitoring systems. Accidental release will be taken care of by cleaning the contaminated area. A thorough investigation of accidental release will be conducted to prevent similar incident from happening in the future. In the extreme event of an accident arising at the field site, all plant materials in the experimental plots will be plowed back into the soil. Any case of sabotage will be immediately reported to the appropriate authorities.

Conditions for Approval:

a) All activities shall be conducted at the Containment Level 2 (CL2) screen house and Isolation Room in the Biotechnology Laboratory of the Philippine Rice Research Institute Central Experimental Station located in Science City of Muñoz, Nueva Ecija.

b) The proponent shall adhere as closely as possible to the schedule of activities reflected in the submitted Gantt chart.

c) Prior to the initiation of the activities, the proponent/s shall discuss the monitoring schedule with the Department of Agriculture-Bureau of Plant Industry-National Plant Quarantine Service Division (DA-BPI-NPQSD) to identify the activities that will require their presence. The proponent/s shall furnish the DOST-BC with a copy of the agreed monitoring schedule.

d) Modifications in the planting schedule and other activities must be communicated to the DOST-BC for information and/or approval.

e) Movement of all transgenic materials should be done in compliance with the DOST-BC and the DA-BPI-NPQSD guidelines.

f) All containment measures should be in place before the actual transplanting of rice seedlings.

g) The proponent must provide a logbook to record access of personnel to the experimental facility.

h) The proponent shall ensure that only the DOST-BC authorized personnel are allowed inside the experimental facilities.

i) The DOST-BC personnel should be informed in advance of any visitations by unauthorized persons.

j) The DOST-BC shall be informed immediately of any intrusion by unauthorized persons.

k) The proponent shall ensure that stray animals are excluded from the experimental facilities while tests are being conducted.

l) In case of undue destruction of experimental materials resulting from unauthorized entry of personnel or breach of containment of experimental facilities, the proponent shall implement measures to prevent the unadvertent escape of any viable material within the facility.

m) The proponent and the supervising IBC shall be held accountable for the undue destruction of the experimental materials and the consequences that their inadvertent escape may cause to the surrounding environment.

n) The proponent shall strictly observe proper disposal procedures for all materials.

o) The rice grains or any plant part shall not be eaten or fed to humans, wildlife and livestock.

p) The proponent shall provide a space within the test site for disposal of the used plant materials.

q) The harvested grains shall be properly labeled and stored at the designated seed storage facility where transgenic seeds are kept, while plant materials and other plant debris used within the test site should be buries in a pit within the test site or plowed under, if applicable.

r) The proponent shall comply with additional requirements that the DOST-BC may impose, as necessary, during the experiment period.

s) The proponent shall submit through the IBC a completion report within 90 days after completion of the experiment.

t) The IBC shall submit to the DOST-BC a report on the completion of this project, in the prescribed format.

Date of Approval (DD-MM-YYYY):
19-11-2016
Date of Completion (DD-MM-YYYY):
Executive Summary:

Golden Rice (GR), which contains elevated levels of the provitamin A beta-carotene in the grains, was developed more than a decade ago through biochemical engineering of the carotenoid biosynthetic pathway. It is intended as an additional food-based strategy to combat the pervasive and persistent problem of vitamin A deficiency (VAD) especially in countries with high rice consumption. VAD can damage the immune system and decrease the body's ability to resist or fight infections; thereby, increasing the risk of mortality from common diseases, especially among women and young children. It may also result in impaired vision, including night blindness, or permanent blindness. In the Philippines, about four out of ten children aged six months to five years and three out of every five lactating mothers have VAD. VAD continues to adversely affect many people despite nutrition interventions such as vitamin A fortification and supplementation programs that seek to provide adequate vitamin A to those at risk. The original Golden Rice donor, Kaybonnet, is a US commercial variety that  grows poorly under Philippine condition, hence, needs to be crossed with local varieties, preferably those with durable resistance to tungro and bacterial blight, two very devastating rice diseases in the country. This project will result in the development of nutrient-enhanced high yielding and disease- resistant rice varieties with excellent agronomic traits (similar to the original local varieties) that can provide beta-carotene to consumers and benefits to farmers.

All research activities on Golden Rice 2-E event (GR2E) (both donor parents and progenies) will be carried out at the Containment Level 2 (CL2) screen house facility of PhilRice-CES, Maligaya, Science City of Muñoz, Nueva Ecija. Strict compliance to biosafety measures and procedures will be implemented at all various stages of crop development.

We propose to conduct the activity from 01 January 2016 to 31 December 2020 at the CL2 screen house, PhilRice-CES, Maligaya, Science City of Muñoz, Nueva Ecija. The main objective of the study is to improve the nutritional value and field performance of different Philippine elite varieties and disease resistant (combination of bacterial leaf blight and tungro resistance) rice variety by incorporating genes for beta-carotene biosynthesis in the rice grains.

Specifically, the objectives of the proposed study are the following:

1. To develop introgression lines with GR genes [phytoene synthase (psy) and carotene desaturase I (crtI)] from high-yielding commercial Philippine rice varieties and disease resistant rice line background through hybridization and marker-assisted selection

2. To fast-track the production of homozygous disease resistant lines through anther culture

3. To validate the resistance of selected lines by tungro and BLB screening

4. To conduct marker-assisted selection to verify that phenotypic data correspond with genotype until stable lines possessing the traits of interest are developed

5. To select elite breeding lines with comparable levels of beta-carotene or better than the donor parent 

Plant Materials

a. Genotypes to be used as donor parents.

    PSB Rc82-GR2-E BC5F4 (IR_112019_GR_2-E:38-4-27 and IR_112019_GR_2-E:38-4-19) and selected BRRI dhan 29-GR2E (BC2F5) lines will be used as source of genes for provitamin A synthesis. PR37171-1-1-1-2-2-1-1 (Line 27;2-in-1) and PR46734-79, which has been confirmed to be resistant to tungro and bacterial blight will be used as disease resistance donors.

b. Genotypes to be used as recurrent parents.

    PSB Rc82, NSIC Rc222, NSIC Rc160, NSIC Rc238, NSIC Rc300, NSIC Rc 298, NSIC Rc308, NSIC Rc354, NSIC Rc358, NSIC Rc402 and IR10M300

c. Check cultivars

    IRBB62 (Xa21, Xa4, Xa7 genes), IRBB4, IRBB5, IRBB7, IRBB21, IRBB61, Matatag 6 (NSIC Rc120; Glh14) ARC11554 (Glh14, tsv1), Utrimerah (tsv1), TN1 and IR 24 (tungro and BB susceptible)

Public Information Sheet

Title Date of Approval Date of Posting File Attachment